EFFECTS OF PROPYLENE GLYCOL MANNATE SUL-FATE(PGMS) ON THE PROLIFERATION OF BOVINE CEREBRAL MICROVESSEL SMOOTH MUSCLE CELLS IN CULTURE
- VernacularTitle:甘糖酯对培养的牛脑微血管平滑肌细胞增殖的影响
- Author:
Xiumei ZHANG
;
Xiaohong SUN
;
Xinbing WEI
- Publication Type:Journal Article
- Keywords:
propylene glycol mannate sulfate (PGMS);
cell culture;
bovine cerebralmicrovessel smooth muscle cells (BCMSMCs);
10% fetal calf serum(FCS);
interleukin-1(IL-1);
cerebral arteriosclerosis
- From:
Chinese Journal of Marine Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The abnormal proliferation of vascular smooth muscle cells after endothe-lial injury is postulated to be the main pathophysiological process in atherosclerosis (AS). The effects of propylene glycol mannate sulfate(PGMS) on the proliferation of bovine cerebral microvessel smooth muscle cslls (BCMSMCs) induced by 10% fetal calf serum (FCS) and interleukin l(IL-1) were investigated in culture. 5 - 8 stage subcultured BCMSMCs were incubated into 96-well dish- With either 10% FCS or IL-1 (50u/ml) to produce BCMSMCs proliferation , the inhibitory effects of PGMS on proliferation of BCMSMCs were investigated. The results shows that PGMS could inhibit the proliferation of quiescent BCMSMCs induced by 10% PCS. The growth of cells was inhibited, comparing with normal control 72hours after the serum addition as determined by crystal violet stainning and MTTmethod. The proliferation of quiescent BCMSMCs induced by IL-1 (50u/ml) was also inhibited by PGMS as determined by crystal violet stainning and MTT method. The results suggested that PGMS inhibit the proliferation of BCMSMCs induced by 10% FCS and IL-1 ,and the use of PGMS may probably play an important role in the treatment of cerebrovascular disease.
