The Effect of Anti-Hepatocellular Carcinoma of HSV-tk Gene Therapy Mediated by Cationic Liposome in vitro and in vivo
- VernacularTitle:阳离子脂质体介导的HSV-tk自杀基因疗法的体内外抗肝癌作用
- Author:
Shuying YANG
;
Fangling DUAN
;
Fengming LU
- Publication Type:Journal Article
- Keywords:
gene therapy;
hepatocellular carcinoma;
herpes simplex virus;
thymidine kinase;
liposome
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the effect of anti-hepato-cellular carcinoma of HSV-tk suicide gene system, we constructed the HSV - tk recombinant retroviral vector pLXT. SMMC - 7721 hepatocellular carconoma cells more transfected with pLXT by lipofectin were obtained by subsequent G418 screen. 3H-TdR incorparation assay showed that HSV - tk/ACV had strong cytotoxic effect on HSV - tk gene transfected tumor cells. Lipofectin pLXT complex was directly injected into murine H22 hepatoma tissue, followed by delivery of ACV prodrup, and it was found that the tumor growth masses more greatly reduced. Animals treated with Liptk + ACV and tk + ACV had an apparent reduction of tumor size as compared with the animals in other six groups ( P
