Influence of Heat Shock Treatment upon the Expression of Membrane HSP70 and Its mRNA of H22 Cell
- VernacularTitle:热休克对H22细胞膜HSP70蛋白及其mRNA水平的影响
- Author:
Weizhong WU
;
Xiaolei TANG
;
Kangda LIU
- Publication Type:Journal Article
- Keywords:
H22 cell;
HSP70;
protein expression;
mRNA transcription
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
To identify the best inducing condition, we studied the expression of membrane protein HSP70 and mRNA of H22 cell at various temperature. Using MTT,RT-PCR, immunofluorescence and FCM techniques, we observed H22 cell survival rate, the expression of HSP70 mRNA and membrane HSP70. No effects of H22 cell survival rate under 42 ~ 43℃ was observed, but cell survival rate declined with increasing stress time at 44 ~ 45 ℃; the level of HSP70 mRNA decreased initially (0.5~4.0) hours but gradually resumed and increased from 8 to 12 hours at 42℃. Membrane HSP70 expressing cells were significently higher in heat shock treatment group than in a control group ( P
