Construction of p16 Gene Recombinant Expression Vector and Its Antitumorigenic Effects on Human Lung Adenocarcinoma Cells
- VernacularTitle:p16基因重组质粒的构建及其对人肺癌细胞的抑制作用
- Author:
Xiaoying FU
;
Shilong CAO
;
Ruiqiong RAN
- Publication Type:Journal Article
- Keywords:
p16 gene;
tumor suppressor gene;
gene therapy
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
p16 gene was a tumor supressor gene found recently. The p16 protein is a negative regulator of cell proliferation . Loss of normal p16 function is associated with the development of neoplasms. To detect antitumorigenic effect of p16 on human lung adenocarcinoma cells, we cloned a p16 cDNA into the pcDNA3 vector at the sites of BamHl and Xho I to gain a p16 gene recombinant expression vector plasmid. We then transferred the p16 gene recombinant plasmid into human lung adenocarcinoma cell line (NCI-H460) by electroporation method. After G418 selection we assessed cell growth properties and cell cycle pattern by flow cytometry to G418-resistant clones of NCI-H460-pl6 and NCI-H460-vect respectively. The results show that human p16 gene could suppress the phenotype of NCI-H460 cell line and p16 gene therapy plays a positive role in human lung adenocarcinoma treatment.
