Inhibition of Foreign Retinoblastoma Gene Mediated by Recombinant Adenovirus Vector on Growth of Bladder Carcinoma Cells
- VernacularTitle:Rb基因导入对膀胱癌细胞生长的抑制作用
- Author:
Hongxia LI
;
Jian LI
;
Yongjing XIA
- Publication Type:Journal Article
- Keywords:
adenovirus vector;
retinoblastoma gene;
bladder carcinoma cell
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the bladder carcinoma cell growth suppression by introduction of foreign retinoblastoma (Rb) gene and explore a gene therapy approach for bladder cancer, a replication-deficient adenovirus vector encoding a wild-type Rb, AdCMVRb, was constructed and transfected into the cultured human bladder carcinoma cell line EJ. The efficiency of gene transfection and expression was detected by immunochemical staining, Western blotting and polymerase chain reaction. The role of Rb in suppressing EJ growth was observed by cell-counting, [3H]thymidine incorporation and flow cytometry. The results showed that wild-type Rb gene could be transfected effectively into cultured EJ with Ad-CMVRb and could arrest the cells at GO/Gl phases of the cell cycle, leading to inhibition of DNA synthesis. The results demonstrated the potential of adenovirus-mediated Rb gene therapy for bladder cancer.
