The Antitumor Effects of Mice Peritoneal Macrophages Transferred IL-2 and IFN-Y Gene in vivo
- VernacularTitle:体内IL-2基因和IFN-?基因转染巨噬细胞抗肿瘤作用的研究
- Author:
Bin ZHOU
;
Zhiyao JIANG
- Publication Type:Journal Article
- Keywords:
gene transfection;
peritoneal macmphage;
antitumor effect;
IL-2 gene;
IFN-y gene
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The eukaryotic expression vector pREP-8-IL-2 or and pREP-8-IFN-? was injected i.p. into mice by calcium phosphate coprecipitation method and IL-2 gene or IFN-? gene was successfully transfected into peritoneal M?s whose expression products could enhance the cytotoxicity of M?s, which secrete some TNF, IL-1 and NO, activate the nonspecific immunity and inhibit the growth of tumor effectively. In particular, IL-2 gene in combination with IFN-? gene transfection were successfully transfected into peritoneal M?s whose high expression products not only significantly enhance the M?s cytotoxicity and made it secrete high level TNF, IL-1 and NO, but also activated the CTLs of spleen and initiated specific immunity and nonspecific immunity . This would produce synergic antitumor effects. The results showed that IL-2 and IFN-? gene transfection produced more antitumor effects than IL-2 or IFN-? gene transfection alone.
