The Effect of rhGM-CSF/IL-3 Fusion Protein on Apoptosis of HL-60 Cells Mediated by Ara-C
- VernacularTitle:rhGM-CSF/IL-3融合蛋白对Ara-C介导的HL-60细胞凋亡的影响
- Author:
Shengming DAI
;
Guiyuan XIAO
;
Shaoxiong ZHOU
- Publication Type:Journal Article
- Keywords:
GM-CSF;
IL-3;
fusion protein;
apoptosis;
HL-60 cell line
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The effect of rhGM-CSF/IL-3 on apoptosis of Ara-C-induced myeloid leukemic cell line HL- 60 was investigated. The results indicated that treatment with rhGM-CSF/IL-3 in combination with Ara-C significantly inhibited the colony growth of HL-60 and enhanced the oligonucleosomal DNA fragmentation as compared with Ara-C alone. The Ara-C mediated apoptosis rates of HL-60 cells treated with rhGM-CSF/IL-3 fusion protein alone were markablely improved compared with treatment with rhIL-3 plus rhGM-CSF, it was noted that the Ara-C mediated of apoptosis normal peripheral white blood cells was less affected by rhGM-CSF/IL-3. It suggested that rhGM-CSF/IL-3 could be used as a possible curing drug during the phase of induced remission of leukemia.
