CONSTRUCTION OF HYBRID NUCLEIC ACID VACCINES BASED ON PLASMODIUM FALCIPARUM MEROZOITE SURFACE PROTEIN1BLOCK17REGION
- VernacularTitle:以恶性疟原虫裂殖子表面蛋白1的17区片段基因为基础构建的复合核酸疫苗候选质粒
- Author:
Jun MIAO
;
Caifang XUE
;
Qigui YU
;
Enqiang QIN
- Publication Type:Journal Article
- Keywords:
N ucleic acid vaccine,m erozoite surface protein1,eukaryotic expression plasmid,T cell epitope,PCR
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To construct hybrid nucleic acid vaccines which contain Plasmodium falciparum merozoite surface protein 1 (MSP1 ) block 1 7gene and gene fragment coding for several T cell epitopes. METHODS:MSP1 block 1 7gene of FUP strain was connected with a polyvalent gene fragment which contains several T cell epitopes from MSA1 ,MSA2 ,RESA,IL- 1 and Tetanus toxin,the connected gene fragment (hybrid gene,HG) was cloned into eukaryotic expression plasmid p CDNA3.1 (- ) ,VR1 0 1 2 for intracellular expression,VR1 0 1 2 /TPA for secretion,the recombinants were identified by PCR and restriction enzyme digestion. RE- SUL TS &CONCLUSION:The hybrid nucieic acid vaccines:VR1 0 1 2 /HG,VR1 0 1 2 /TPA/ HG and p CDNA3.1 /HG were successfully constructed.
