Specific Cytotoxic T Lymphocytes Responses Induced by Colorectal Cancer Antigen CA-Hb3 in vitro
- VernacularTitle:大肠癌CA-Hb_3抗原体外可诱导特异性CTL反应
- Author:
Hongbing GUAN
;
Wenqian WEN
;
Jinqiu HUANG
- Publication Type:Journal Article
- Keywords:
colorectal cancer;
liposome;
soluble antigen;
cytotoxic T lymphocytes
- From:
Chinese Journal of Cancer Biotherapy
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
CTL responses by autologous colorectal cancer cells were observed in five cases. The specific CTL was induced from two cases, in which tumor associated antigen, CA-Hb_3, was strongly positive. The CTL could not induced from other three cases in which CA-Hb_3 antigen was weakly positive. Weakly positive tumor cells in the three cases were loaded CA-Hb_3 antigen into cytoplasmas by the cationic liposomes and tested by immunohistochemical assay. The results showed that CA-Hb_3 antigen of tumor cells in two cases changed to strongly positive, and could induce CTL responses successfully. With blocking agents BFA and anti-MHC-I monoclonal antibody, CTL responses could be blocked. Our results suggested that soluble CA-Hb_3 antigen could enhance the immunogenity of colorectal cancer cells and induce effective anti-tumor cellular immune responses.
