In vitro Proliferation and Cytotoxicity of PBMC Induced by Autologous Tumor Specific Antigen Costimulated with IL-2
- VernacularTitle:自体可溶性肿瘤抗原协同IL-2诱导外周血单个核细胞体外增殖及杀瘤作用的研究
- Author:
Yuanmei LIN
;
Zhenhua LIU
- Publication Type:Journal Article
- Keywords:
autologous soluble tumor antigen;
interleukin-2;
cell proliferation;
cytotoxicity
- From:
Chinese Journal of Cancer Biotherapy
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
To observe its proliferation and cytolytic activity in vitro induced by autologous soluble tumor antigen and IL-2, PBMC costimulated with TSA extracted from gastric tumor by biochemical procedure and IL-2 was cultured. The proliferation and cytotoxicity were evaluated by MTT method and the phenotype was analysed by APAAP method. TSA alone did not induce PBMC proliferation while TSA (10?g/ml) with IL-2 presented syngenic stimulating effect which was time and concentration dependent. The cytolytic unit of PBMC induced with IL-2/TSA was 86.3% vs 48.6% with IL-2 alone. The phenotypic measurement demonstrated that TSA enhanced CD25 and CD8 expression significantly. The observation offered experimental data for further research work about tumor activated killer(TAK) cells. The results indicated that TAK cells would be a new type of immunoactive cells in adoptive immunotherapy against tumor.
