INVESTIGATION OF THE SPECIESSTATUSOF ANOPHELES DIRUS( DIPTERA:CULICIDAE) FROM HAINAN PROVINCE USING r DNA
- VernacularTitle:用rDNA研究海南省大劣按蚊的种型分类地位
- Author:
Jiannong XU
;
Fengyi QU
- Publication Type:Journal Article
- Keywords:
Anop heles dirus,ribosom al DNA,second internal transcribed spacer
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1997;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To ascertain the species status of the Anopheles dirus from Hainan Province,Chi- na.METHODS:The nucleotide sequence of the second internal transcribed spacer(ITS2 ) of PCR- amplified r DNA was determined for the An.dirusspecimens which included 2 individu- als of the species A colony (AFRIMS) from Thailand and 5 individuals from Hainan Province.RESUL TS:A841 bp fragment was amplified from single mosquito.The fragment included the ITS2 and small portions of flanking 5 .8S (96 bp) and 2 8S (2 9bp) genes.The ITS2 was71 6 bp in length,the sequence was identical for all7individuals from both Hainan Province and Thailand.No evidence of intraspecies or intrapopulation variation was detected in the ITS2 and flanking regions.CONCL USION:The result suggests the existence of An. dirus A in Hainan Province,which was in agreement with previous studies from cytogenetic analysis and egg characteristics determined by scanning electron microscopy.
