Effect of Retroviral Vector-Mediated IL6/IL2 Fused Gene Transduction on B16 Melanoma Cells Adhesive and Metastatic Potential
- VernacularTitle:逆转录病毒介导的IL6/IL2融合基因转导对小鼠B16细胞粘附及转移特性的影响
- Author:
Xiaodan YU
;
Peixian TANG
;
Mingwei ZHANG
- Publication Type:Journal Article
- Keywords:
cytokines;
fusion gene;
gene transfer;
tumor;
metastasis
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to examine the feasibility of cytokine fusion gene transfer into tumor cells, a retroviral vector (pLXSN) for human interleukin 6/interleukin 2 (IL6/IL2) fusion gene was constructed by using PCR and ligating the 2 genes with a synthesized flexible linker. The IL6/IL2 fused gene was introduced into B16 melanoma cell line mediated by retrovirus and the changes of adhesive and metastatic ability of fused gene-modified cells were detected. The B16-IL6/ IL2 cells showed efficient expression of both cytokines and decreased binding affinity with extracellular matrix (Laminin and Matrigel), accompanying with decreased experimental metastatic potentials. These data suggest that the mechanism involving the decreased metastases may relate with the changes of biological characteristics and immune stimulating activity of gene-modified cells.
