Efficient Generation of Human B7(CD80) Recombinant Adenovirus by COS/TPC Homologous Recombination
- VernacularTitle:COS/TPC同源重组法高效制备人B7(CD80)重组腺病毒
- Author:
Weiping ZHANG
;
Xuetao CAO
;
Hirofumi HAMADA
- Publication Type:Journal Article
- Keywords:
costimulatory molecules;
B7(CD80);
adenovirus vector;
gene transfer;
gene expression
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The human CD80 full-length encoding cDNA was cloned by RT-PCR, and inserted into El-substituted aden-ovirus vector pAxlcw. Subsequently, the hCD80 recombinant adenovirus vector was cotransfected into 293 cells together with EcoT22I-digested Ad5-TPC, and the replication-deficient hCD80 recombinant adenovirus was generated efficiently by homologous recombination, with the tilers of 4?10~9pfu/ml. Infected with prepared hCD80 recombinant adenovirus in vitro, Hela cells expressed high levels of hCD80 48 hours after infection. These suggest that COS/TPC is an efficient method to prepare recombinant adenoviruses and the prepared hCD80 adenovirus could be used in cancer gene therapy.
