Antitumor Study of Human Tumor Necrosis Factor Alpha Gene Modified Fibroblasts
- VernacularTitle:人TNF-?基因修饰成纤维细胞的抗肿瘤研究
- Author:
Guanhua LAI
;
Zhanyun TANG
;
Shishu CHEN
- Publication Type:Journal Article
- Keywords:
tumor necrosis factor;
fibroblasts;
gene therapy;
bicistronic retrovirus vector
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the antitumor effect of human tumor necrosis factor alpha (TNF-?)gene modified fibroblasts, we have transfered TNF-? cDNA to fibroblasts NIH3T3 by a bicistronic retroviral vector pGCEN/TNF-?, which expressed TNF-? from the long terminal repeat and the selectable Neo~R from the encephalomyocarditis virus (EMCV) internal ribo-some entry site(IRES) . The results indicated that TNF-? modified NTH3T3, NIH3T3/TNF-? ,were able to express TNF-? at a high level for a long term culture. Furthermore, these modified fibroblasts could inhibit tumor progression signifi-cantly(P
