Suppression of Gastric Cancer Cell Line Growth After Transfection of TNF-a Gene
- VernacularTitle:TNF-?基因转染对胃癌细胞系的生长抑制效应
- Author:
Wei ZOU
;
Lijun WANG
;
Naiheng ZHANG
- Publication Type:Journal Article
- Keywords:
gene;
TNF-a;
cell line;
gastric cancer;
gene therapy
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Human gastric cancer cell line MKN28 and BGC823 were infected with recombinated retroviruses containing TNF-? gene, The expression and function of TNF were identified in transfected cancer cell line MKN28-TNF and BGC823-TNF. Cancer cells showed lower growth rate after transfection, and the inhibition of growth rate were 71% and 66% in MKN28-TNF and BGC823-TNF in comparison with the control, respectively. Decreased levels of ~3 HTdR incorporation were observed. The tumorigenicity of MKN28-TNF in nude mice was completely lost as compared with the control . The results demonstrate the inhibition of gastric cancer cells growth and tumorigenicity introduced by exogenous TNF gene.
