The Study of Inhibition of Malignant Phenotype of SAC-IIB2 Cell Strains by Antisense Oligodeoxynucleotide of c-fos
- VernacularTitle:反义c-fos寡脱氧核苷酸对淋巴瘤细胞株SAC-IIB_2恶性表型抑制的研究
- Author:
Kaiping YAN
;
Lianhua YIN
;
Xianshou KONG
- Publication Type:Journal Article
- Keywords:
lymphoma;
cell strains;
c-fos;
antisense oligodeoxynucleotides;
gene therapy
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
It was found that protooncogene c-fos was over-expressing in SAC-IIB_2 cell strains. Targeting the code 2-7 sequence of c-fos cDNA of mice, we designed and synthesised eighteen mers antisense. sense and nonsense oligodeoxynucleotides(ODN). The results indicated that antisense c-fos ODN could induce SAC- IIB_2 maturative differentiation, inhibit its growth rate, decrease its ability of forming tumor in nude mice and expression of Fos protein, however, antisense ODN has no remarkable effect on cellular dynamics of SAC-IIB2. But sense and nonsense c-fos ODN have no similar effect on SAC-IIB_2 cell strains. The results demonstrated that antisense c-fos oligodeoxynucleotide has inhibition effects on malignant phenotype of mice SRS lymphoma cell.
