The Biological Characteristics of IL-2 Gene Modified Tumor Cells
- VernacularTitle:白细胞介素2基因转染肿瘤细胞体外生物学特性的研究
- Author:
Yihua SUN
;
Dianjun LI
;
Zhihua WANG
- Publication Type:Journal Article
- Keywords:
gene therapy;
tumor;
interleukin-2;
retrovirus vector
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
In this experiment, we introduced human interleukin-2(lL-2)gene and Neomycin resistance (Neo~R) gene into a human lung adenocarcinoma cell line GLC, using a retroviral vector PLXSN. The positive cells obtained by selecting in G418 were compared with the wild-type cells. We observed their growth characteristics in vitro and the quantity of IL-2 in the culture supernatants. PCR result demonstrated the integration of foreign genes into tumor genome DNA after transfection and permanent existence. Under electron microscope, what can be seen was the surface villi of trans-duced-cells are less and shorter. It deserves further investigation to determine whether the change of cell surface influence metastatic ability of cells.
