The Effect of Antisense IGF-I Gene on the Tumor Immunogenicity of Human Hepatoma 7402 Cell
- VernacularTitle:反义IGF-I基因对人7402肝癌细胞肿瘤免疫原性影响的研究
- Author:
Li ZHANG
;
Shunong LI
;
Xiaoning WANG
- Publication Type:Journal Article
- Keywords:
antisense IGF-1;
tumor immunogenicity;
MHC;
LAK
- From:
Chinese Journal of Cancer Biotherapy
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
In this research, we apply the antisense strategy to study the function of IGF- I in human hepatoma lines(7402) by Lipofectin mediated gene transfer. The effect of antisense IGF-I on the expression of MHC and the sensitivity of LAK cytotoxicity of tumor cell were studied. The results showed that human hepotoma lines(7402) were transfected with an amplifiable antisense IGF- I gene. The expression antisense IGF-I RNA in the transfected cells after hygromycin resistant selection was confirmed by northern blot analysis. The transfectants expressed higher level of MHC class I and class II molecules. The susceptibility of the transfected cell to LAK cytotoxicity was greater than that of 7402 parent cells(P
