In vitro Biological Characteristic Changes and in vivo Tumorigenicity of mlL-4 Gene Modified G422 Cells by Adenovirus-Mediated Gene Transfer
- VernacularTitle:重组腺病毒介导mIL-4基因转染的G422胶质母细胞瘤体外生物学特性及体内致瘤性的改变
- Author:
Bo HONG
;
Xuetao CAO
;
Cheng ZHU
- Publication Type:Journal Article
- Keywords:
glioma;
interleukin-4;
gene therapy;
adenovirus
- From:
Chinese Journal of Cancer Biotherapy
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Murine interleukin-4 gene(mIL-4) was transfected into mice glioblastoma cell line G422 by recombinant adenovirus vector. We detected mRNA transcription of target gene in gene modified tumor cells(G422-mIL4). High level of mouse IL-4 could be detected in the culture supernatant of G422-mIL4. When inoculated subcuatneously, the tumor growth of G422-mIL4 was significantly inhibited as compared to wild type G422 and LacZ gene modified G422( G422-LacZ) . The period of survival of mice inoculated with G422-mlL4 was significantly prolongated(p
