A methodologic study on the determination of islet cell antibodies
- VernacularTitle:胰岛细胞抗体检测方法研究
- Author:
Yongde PENG
;
Yuanfeng HU
;
Zhifang GU
- Publication Type:Journal Article
- Keywords:
ICA;
Immunolabelling;
Immunofluorescence
- From:
Chinese Journal of Diabetes
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
To exclude the interfering factors in islet cell antibodies(ICA) determination some pretreatments on both pancreatic sections and serum samples were made. The results revealed that the islet structures of pretreated pancreatic frozen section appeared clearly. The positive rate of ICA by ABC enzyme-labelled method was higher than that by indirect immunofluorescence method. Comparing the ICA results of exchange sera provided by Immunology and Diabetes Workshop with the reference results,the validity、consistency、sensitivity and specificity was 81%、63%、75%and 88%, respectively. These results suggest that ABC method with the pretreated pancreatic frozen section is a stable method of ICA determination.
