Cytotoxicity of Human Tumor Draining Lymph Node Cells Stimulated by Cancer-Associated Apomucin in vitro
- VernacularTitle:癌相关抗原粘蛋白前体诱导人肿瘤引流区淋巴结的细胞毒作用
- Author:
Yunguo MA
;
Mei YUAN
;
Li LI
- Publication Type:Journal Article
- Keywords:
apomucin;
adenocarcinoma;
anti-tumor immunotherapy
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Human tumor antigen elicit a cell-mediate immune response in the host have been identified. In this study, a pancreatic cancer cell line SW1990 associated apomucin was examined for the induction of tumor draining lymph node cells(TDLN) anti-tumor immune responses.The TDLN of patients with breast cancer(4 cases), gastric cancer(3 cases), colon cancer(3 cases) were isolated, cocultured with apomucin 50 ?g/ml and low dose rhIL-2 50U/ml in vitro, the cytolytic effects were measured by MTT assay. The results showed that: the proliferated lymphocytes can lysis LS174T colon cancer cells, KATO-Ⅲ gastric cancer cells, SW1990 pancreatic cancer cells and K562 leukemia cells. The most cytolytic effects were higher than LAK. This results might provide a rational basis for future attempts to elicit and manipulate apomucin vaccine for active specific immunotherapy or adoptive immunotherapy for adenocarcinoma.
