The Effects of Parvoviral Nonstructural Proteins Gene Transfection on the Expression of Cytokines in Gastric Cancer Cell
- VernacularTitle:细小病毒非结构蛋白基因转染对胃癌细胞株细胞因子表达的影响
- Author:
Xizhong SHEN
;
Guanhua YU
;
Shaoji JIANG
- Publication Type:Journal Article
- Keywords:
parvoviral nonstructural proteins;
gene transfection;
cytokine.
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Human gastric cancer cells (MKN-45) were transfected with pULB3238 (a plasmid carrying MVMp NS gene). After the NS gene expressed, some of the NS-transfectants were died. The other cells were survived. In comparison with those of control MKN-45, the survived NS- transfected MKN-45 cells had the following characterizations: (a). nucleus/cytoplasm ratio reduced; (b). generation time prolonged;(c). adhesion ability between cells increased;(d). cloning efficiency decreased;(e). formation of tumors in nude mice suppressed. To investigate the possible mechanisms of above mentioned change of NS-transfectants, the effect of NS on the expression of some cytokine genes were explored. RNA dot blot indicated that the NS might enhance the expression of IL-l?(2.7 times), IL-1/?(4.6 times) IL- 6 nuclear factor (2.5 times) and had no effect on the expression of IL-6. The results suggested that the antineoplastic activity of parvoviruses may be medicated by NS interfere with the expression of cytokines in the host cells.
