Various Amounts of rhGM - CSF cDNA Transfer Directly into Mice's Muscle in vivo
- VernacularTitle:不同剂量rhGM-CSF cDNA直接注射小鼠骨骼肌的表达
- Author:
Shu ZHANG
;
Qinong YE
;
Ruihua ZHAO
- Publication Type:Journal Article
- Keywords:
gene therapy;
GM - CSF;
gene transfer;
skeletal muscle
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
We have transferred the different amounts of the plasmid DNA pCGl, which was constructed by cloning rhGM-CSF gene into the eukaryotic expression plasmid pCDS, directly into the BALB/c mice's quadriceps pretreated with bupivacaine. The result of ELISA confirmed that rhGM - CSF was expressed and secreted in mice of group B injected 60?g of plasmid pCGl, but not in mice of group C injected 30?g of plasmid pCGl. The time for the highest level of rhGM-CSF expression was during the fifteenth to twentieth days after plasmid DNA pCGl was injected. Meanwhile, the results of biological activity detection showed that mice's serum of group B (injected 60?g) had the function of retaining the growth of TF - 1 cells. This demonstrates that the rhGM-CSF in mice's serum of group B has certain biological activity. This Model of animal will blaze a new trail in the treatment and prevention of the leukyopenia.
