IDENTIFICATION OF GLYCOSAMINOGLYCAN FROM THE BAY SCALLOP ARGOPECTEN IRRADIAUS BY AGAROSE GEL ELEC-TROPHORESIS
- VernacularTitle:扇贝糖胺聚糖的琼脂糖凝胶电脉分析
- Author:
Changyun WANG
;
Huashi GUAN
;
Shuqing LIU
- Publication Type:Journal Article
- Keywords:
Bay scallop (Argopecten irradiaus );
glycosaminoglycan;
agarose gel elec-trophoresis;
analysis
- From:
Chinese Journal of Marine Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Fraction F3-3-3 of glycosaminoglycan was isolated from the leftover bits of Bay scallop Argopecten irradiaus . Infrared spectra of F3-3-3 was similar to that of heparin. Agarose gel electrophoresis of this fraction was performed in the system of barbital buffer (0. 06 mol/L,pH 8. 6). It was found that F3-3-3 showed single band in the electrophoresis. Compared with the agarose gel electrophoresis of standard glycosaminoglycan, such as heparin, hyaluronic acid and chondroitin 6-sulfate, it indicated that the relative migration rate of the fraction was close to that of heparin.
