Effects of G-CSF Gene Therapy bn the Number and Functions of Peripheral Neutrophils of Colon Adenocarcinoma-Bearing Mice Receiving High-Dose Chemotherapy
- VernacularTitle:G-CSP基因治疗对大剂量化疗后结肠癌小鼠中性粒细胞数量和功能的影响
- Author:
Yanping SUN
;
Xuetao CAO
;
Quanxing WANG
- Publication Type:Journal Article
- Keywords:
granulocyte colony - stimulating factor;
gene therapy;
colon adenocarcinoma;
neutrophil;
5 - fluorouracil;
cytotoxicity;
cytokine
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Neutrophils play important role in anti - tumor response of tumor-bearing host as a kind of important effector cells. In order to identify the anti-tumor mechanisms of G-CSF gene therapy, we investigated the number and functions of the peripheral neutrophils in the C-26 colon adenocarcinoma-bearing mice receiving G - CSF gene therapy and high - dose chemotherapy. After G - CSF gene therapy, the number of neutrophils in peripheral blood was increased markedly in C-26 mice receiving high - dose 5 - Fu as compared with control groups including in vivo administration of rhG - CSF. The more potent cytotoxicity to C - 26 cells could be detected. The phagocytic activity, the secretion of IL-1, TNF, NO of the neutrophils were significantly enhanced. These data showed that G - CSF gene therapy can increase the number of neutrophils, activate the functions more effectively than in vivo administration of rhG - CSF.
