The Novel Procedures to Increase the Expression Efficiency of Recombinant Human IL-6 in Eukaryotic Cells
- VernacularTitle:提高人重组IL-6在真核细胞中表达的新途径
- Author:
Hongtao LIU
;
Yong YUAN
;
Yingmei ZHANG
- Publication Type:Journal Article
- Keywords:
Interleukin 6;
gene transfection;
intron;
COS-7 cell
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to improve the expression efficiency of recombinant IL~6 in eukaryotic cells, we constructed a new vector pcDIIL-6 to express IL-6 in eukaryotic cells on the basis of an eukaryotic vector pcDNA3IL-6 by introducin g the first intron of human?-globin gene into the downstream of CMV promoter in the pcDNASIL - 6. The IL-6 level expressed by pcDIIL - 6 is 7. 5 times higher than that of pcDNA3IL - 6. On the other hand , we studied the influence of pAdVAntage vector co - transfection on the expression level of IL-6 in eukaryotic cells and found the level of IL-6 is 3. 6 times higher than that of pcDNA3IL - 6. The results we got are beneficial to express cytokines with high efficiency in eukaryotic cells.
