Role of CD4S in NK Cells Activation and the Molecular Mechanism
- VernacularTitle:CD45分子在NK细胞激活中的作用及其分子机制研究
- Author:
Feng SHEN
;
Mengchao WU
;
Han CHEN
- Publication Type:Journal Article
- Keywords:
NK cells;
CD45;
signal transduction
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
For identifying the molecules that trigger NK cell activation and investigating the mechanism by which the identified molecule mediates NK cell signal transduction, NK cells was stimulated with mAbs specific for a series of adhesion molecules their ligands and transfectants. IFN-gamma production by NK cells was quantitated. We first report here that crosslinking of CD45 specifically stimulates INF-gamma production in NK cells, and such effect was independent of CD16. NK cells gradually lose expression of CD45RA upon culture with IL-2 and concomitantly acquire the expression of CD45RO. CD45RO, rather than CD45RA, play stimulating roles in INF-gamma production in NK cells. Whereas NK cells bound to CD22? and CD22? transfectants, this interaction was not able to stimulate NK cells to secrete IFN - gamma. Our observation suggests that CD45 crosslinking, as an independent and unique stimuli, mediates NK cell signaling.
