Mood Group Serological Identification of Monocolonal Antibodies Anti-M and Anti-N
- VernacularTitle:两株抗M和两株抗N单克隆抗体的血型血清学分析
- Author:
Shizhong LI
- Publication Type:Journal Article
- Keywords:
anti-M;
anti-N;
McAb
- From:
Chinese Journal of Blood Transfusion
1988;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The BALB/c mice were immunized with human O blood cells. Their spleen cells were fused with murine myeloma cells Ns-1 with PEG. The monoclonal antibodies were identified with human panelled cells. Two clones secreting monoclonal anti-M(2Al and 3F6)and another two clones secreting monoclonal anti-N(4E3 and 7G5)were identified. All these clones could consistently secrete IgM antibodies. In typing 300 different RBC samples,the specificity of antibodies were identical to those of commercial humen anti-M and anti-N typing sere reagent. Significantly, anti-N reagent 4E3 differed from anti-N reagent 7G5, which the former one couldn't be absorbed by M erythrocytes at all,while the latter one absorbed by M erythroeytes.
