Preparation and Anti-tumor Activity of Adherent LAK Cells from Patients with Hepatocellular Carcinoma
- VernacularTitle:肝癌患者粘附性LAK细胞制备及抗瘤活性研究
- Author:
Xiaojie CHAI
;
Shenlong YE
;
Weizhong WU
- Publication Type:Journal Article
- Keywords:
adherent LAK cells (A-LAK);
phenylalanine methyl ester (PME);
hepatocellular carcinoma (HCC)
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Highly active adherent LAK cells (A-LAK) with monocytes depleted by phenylalahine methyl ester (PME) were cultured from peripheral blood lymphocytes of patients with hepatocellular carcinoma (HCC). Results showed that A-LAK cells cultured in about 14 days had expanded better and faster than that of nonadherent LAK cells (NA-LAK) with their greatest expansion varied from 23 to 243 fold .A-LAK cells showed a trend of increase in TH cell subgroup and decrease in Ts cell sub-group as well as significant difference of TH/TS ratio. The IL-2R expression increased from 34.1%to 64.3%. A-LAK cells had a higher cytotoxicity (64.6%)than that of NA-LAK cells (42.8%).Further clinical application of A-LAK cells may improve biotherapeutic effect on HCC patients compared with that of NA-LAK cells .
