In vitro and in vivo Cytokine Gene Transfer Mediated by Liposome
- VernacularTitle:脂质体介导的体内外细胞因子基因转移效果的初步研究
- Author:
Quanxing WANG
;
Xuetao CAO
;
Weiping ZHANG
- Publication Type:Journal Article
- Keywords:
liposome;
gene transfer;
interleukin-2;
interleukin-6;
gene therapy
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
In the present study, we prepared cationic liposomes encapsulated IL-2 DNA or IL-6 DNA with reverse-phase evaporation to investigate their efficiency in mediating gene transfer. After transfection with IL-2 DNA liposome or IL-6 DNA liposome, the supernatants of NIH3T3 cell contained high levels of IL-2 or IL-6. The peritoneal cells from the mice injected i.p. of IL-2 DNA or IL-6 DNA liposome 10 days later could also be detected to secrete IL-2 or IL-6. The B16F10 melanoma cells from tumor-bearing mice which were injected intratumorally of IL-2 DNA or IL-6 DNA liposome exhibited resistant to G418 and secreted high levels of IL-2 or IL-6. The results show that the liposome can mediate cytokine gene transfer efficiently in vitro and in vivo.
