Study on PA317 Producing High Titers of Retroviral Vector Inserted with Human TNF-?Gene
- VernacularTitle:PA317产生高滴度带有人TNF-?基因逆转录病毒的研究
- Author:
Liang HU
;
Guanxiang QIAN
;
Wenhong SHEN
- Publication Type:Journal Article
- Keywords:
retrovirus-mediated gene transfer;
amphotropic retrovirus packaging cell;
defective retroviral vector
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to use retroviral-mediated gene transfer technology in clinical application, retroviral vector must be of high titer and free of detectable replication-competent retroviruses (RCR). The aim of this study was to optimize methods of defective retroviral vector production. Study was conducted using a LXSN vector inserted with human tumor necrosis factor-? gene and an amphotropic retrovirus packaging cell line-PA317. The results indicated that viral titer was influenced by volume of medium and concentration of fetal calf serum. Inactivation of retroviral vector was greater at 37癈 than at 32癈. In experiment of transfection of PA317 and transduction of 3T3, integration of retroviral vector into genome of packaging cells and target cells, and free of RCR were detected by polymerase chain reaction analysis. Viral vector with high titer and free of RCR is able to use in clinical trial
