Antitumor Activity of the Conjugates Composed of C1027, an Anticancer Antibiotic, and Monoclonal Antibody
- VernacularTitle:抗癌抗生素C1027与单克隆抗体偶联物的抗肿瘤作用
- Author:
Yongsu ZHEN
;
Rongguang SHAO
;
Min JIANG
- Publication Type:Journal Article
- Keywords:
anticancer antibiotic C1027;
enediyne;
immunoconjugate
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
C1027, an anticancer antibiotic, showed extremely potent cytotoxicity against cultured cancer cells, being over 10,000-fold more potent than adriamycin. C1027 acted rapidly; DNA synthesis of hepatoma cells was suppressed within 10 mintus of exposure. C1027 consists of two moieties, a new enediyne chromophore and an apoprotein; the chromophore serves as the active part of the molecule. C1027 molecule can be dissociated and reconstituted. The reconstituted C1027 was as potent as natural C1027. Through a newly developed method, enriched conjugation C1027 was conjugated to monoclonal antibodies. As determined by clonogenic assay, the conjugates displayed highly-potent, specific cytotoxicity to target cancer cells. The conjugates exerted marked therapeutic effects against hepatoma and gastric cancer xenografts in nude mice; at tolerable dose, tumor inhibition rate reached 85% (P
