Targeting T Lymphocytes by Bi-specific Monoclonal Antibodies to Eliminate Tumor Cells in Bone Marrow
- VernacularTitle:双特异抗体导向骨髓中活化T细胞清除骨髓中肿瘤细胞
- Author:
Kunyuan GUO
;
Senming WANG
;
Guoqiang CHEN
- Publication Type:Journal Article
- Keywords:
purging bone marrow cell;
lung carcinoma;
bi-specific monoclonal antibody;
T cell
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
PC84045 human lung carcinoma cells (PC84045 HLCCs) were mixed with the BM cells activated by anti-CD3 McAb and IL-2 and then CD3-ALTO4 bi-specific McAbs was put into the cell suspension. The mixed cells were cultured for 3 days in presence of IL-2 and IL-3. Tumor cell colony formation method in half-solid culture system was used to assess the purging efficiency (PE) of the activated T cells to PC84045 HLCCs. The ratio of T cells to PC84045 HLCCs was 8 to 1, the PE was 4 Logs. The rario of T cells to PC84045 HLCCs was 16 tol, the PE was 5 Logs. When the purged BM cells were injected into the NC nude mice, none of subcutaneous node was found. The number of the CFU-GM, BFU-E of the purged BM cells was more than 85 % compared with that of the fresh BM cells (P
