Construction of Anti-CD3 Human/Murine Chimeric Antibody Genes for Expression in Mammalian Cells
- VernacularTitle:抗人CD3人/鼠嵌合抗体基因的构建
- Author:
Zhihua YANG
;
Yiwan LI
;
Decheng SHEN
- Publication Type:Journal Article
- Keywords:
anti-CD3 antibody;
polymerase chain reaction(PCR);
gene library;
gene cloning;
chimeric antibody gene
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
For construction of anti-CD3 human/murine chimeric antibody genes, a selective first-strand cDNA synthesis from mRNA or RNA of murine McAb HIT3a was performed using murine Ig constant region primers, and then cDNA of heavy and light chain variable domains of murine immunoglobulin were amplified by PCR using a set of degenerated oligonucleotide primers. Using these cDNA fragments as probes, the L and H chain V region exons encoding the murine McAb anti-CD3 were isolated from the gene library of HIT3a DNA and inserted into mammalian expression vectors containing the human ? and yl constant region exons for construction of human/murine chimeric antibody genes.
