Preliminary Report on Preparation of Monoclonal Antibodies to Human Plasma Protein C
- VernacularTitle:抗人血浆蛋白C单克隆抗体研制的初步报告
- Author:
Hailing WANG
- Publication Type:Journal Article
- Keywords:
Human plasma protein C;
Monoclonal antibody;
Divalent metal ion;
Enzymatic activity
- From:
Chinese Journal of Blood Transfusion
1988;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Eight cell strains of mouse hybridoma named PC 01 to PC 08 have been produced by immu-'nizing the mice with human plasma protein C (HPC) which is purified using physico-chemical method. Each of them has long-term stable ability to secret the monoclonal antibodies. Among them,PC 03 is a conformation-specific monoclonal antibody,and its binding to HPC can be pro-motad distinctly by divalent metal ion. However,the monoclonal antibodies of PC02 and PC03 can noticeably advance the enzymatic activity of HPC,whereas the monoclonal antibody of PC04 has capacity to inhibit the enzymatic activity of HPC.
