Preliminary Study on Transduction of Tumor-infiltrating Lymphocyte with Tumor Necrosis Factor Gene and Its Clinical Trial
- VernacularTitle:肿瘤坏死因子基因转导肿瘤浸润淋巴细胞的初步研究及应用
- Author:
Jianqing DING
;
Guanxiang QIAN
;
Biaoru LI
- Publication Type:Journal Article
- Keywords:
tumor-infiltrating lymphocyte;
tumor necrosis factor gene;
transduction
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
We selected defective retrovirus LXSN approved by the recombinant DNA Advisory Comittee, NIH, USA as a vector to study the transduction of human TIL with TNF gene, on the basis of which, we engaged in the clinical application. The results showed that the logarithmic growth phase was the optimal transducing time when the TIL were isolated and cultured from 9 to 25 days. It also showed that the transduction efficiency was correlated to the MOI value, the more MOI value, the more TIL being transduced. Successful gene inserted and expressing were confirmed by the polymerase chain reaction and the L929 cell test. The clinical trial of treatment for metastastic hepatic cancer suggested that the better therapeutic effectiveness and less side effect depend on the specificity of the TIL, the proper infusion pathway and the dose of the transduced TIL.
