Construction of ODC Antisense RNA Expression Piasmid and Its Effect on the Growth Characteristics of Human Lung Squamous Carcinoma Cells
- VernacularTitle:ODC反义RNA表达质粒的构建及其对人肺鳞癌细胞生长特性的影响
- Author:
Jun GUAN
;
Muzhen FAN
;
Shulan CAO
- Publication Type:Journal Article
- Keywords:
ornithine decarboxylase;
antisense ribonucleic acid;
lung squamous cell carcinoma
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Orinthine decarboxylase (ODC) is a rate-limiting enzyme in polyamine biosynthesis. Abnormal elevation of ODC activity and subsequent polyamine accumulation are intimately associated with the genesis, development and metastasis of cancer. ODC antisense RNA expression plasmid, pCMV/ODCas, was constructed, with which the cells of human lung squamous carcinoma were transfected by the calcium phosphate precipitation method. After G418 selection, two G418 resistant colonies were obtained. The growth rate, the ability to form colony in soft agar, the biosyntheses of DNA, RNA and proteins, and ODC activities of the two antisense transfectants were significantly suppressed in comparison with the parental cell line. It was demonstrated that these changes were associated with the stable integration of exogenous antisense ODC gene in the genomic DNA of transfectants by analysis of Southern blot and PCR.
