Therapeutic Effect of TNF Gene-transfected LAK Cells on the Ascitic Liver Carcinoma-bearing Mice
- VernacularTitle:腹腔内注射TNF基因转染的LAK细胞对于腹水型肝癌小鼠的疗效
- Author:
Guoliang LOU
;
Xuetao CAO
;
Bihe MIN
- Publication Type:Journal Article
- Keywords:
tumor necrosis factor;
gene transfection;
gene therapy;
LAK cells;
liver carcinoma
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
TNF gene was transfected into murine LAK cells by retrovirus. Our results showed that TNF gene-transfected LAK cells secreted TNF more than normal LAK cells and control gehe-transfected LAK cells. The in vitro growth ability and cytotoxicity of TNF gene-transfected LAK cells were augmented significantly.The cytotoxicity of ,TNF gene-transfected LAK cells was markedly inhibited by anti - TNF monoclonal antibody, indicating that the, above augmentation was mediated by TNF secreted by transfected LAK cells. Significant therapeutic effect on the ascitic liver carcinoma.-bearing mice was achieved by i.p. injection of low dosage TNF gene transfected LAK cells and IL - 2.
