Construction of c-myc RNA Antisense Expression Plasmid and Its Effects on the Malignant Phenotype of HL60 Cell Line
- VernacularTitle:c—myc反义RNA转录体的构建及其对HL60细胞恶性表型的影响
- Author:
Yinxiong LI
;
Muzhen FAN
;
Jingli ZHANG
- Publication Type:Journal Article
- Keywords:
c-myc;
antisense RNA;
HL60;
gene transfer
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The inducible c-myc antisense RNA expression plasmid PGXC expressed in mammalian cells was constructed. PGXC was cotransferred with plasmid pSV2neo which confers resistance to Geneticin (G418) to human promyclocytic leukemia cell line HL60. Following the promotion of c-myc antisense RNA expression with Cd~(2+) the growth rate of transfectant HL_(60)~R-9 was significantly slower than that of the parent cells HL60. Flow cytometric analysis indicated a marked increase in Gl fraction and decrease in S-phase population in the cell cycle of transfectants. The differentiation of cellular morphology and function of HL_(60)~R-9 was induced. HL_(60)~R-9 did not form colonies on soft agar and had reduced or lost the tumorigenicity in nude mice.
