Studies on the Apoptotic Cell Death of Interleukin-2 Gene Modified Mouse Hepatoma Cells
- VernacularTitle:IL—2基因修饰鼠肝癌细胞引起的凋亡研究
- Author:
Tengfei ZHANG
;
Yuxiao SUN
;
Shishu CHEN
- Publication Type:Journal Article
- Keywords:
human IL ?2 gene;
retroviral vector;
gene transfected;
apoptosis.
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Retroviral vector was used to introduce the interleukin - 2 (IL -2) gene into H22 cells, a BALB/c mouse hepatoma cell line. The IL-2 gene and marker gene (NeoR) were assessed by using PCR and RT-PCR methods. FACS analysis demonstrated that the cells with low DNA content in IL-2 gene modified H22 cells were more than these in control H22 cells. Electron microscopic morphological structure showed that some cells in IL-2 gene modified H22 went into apoptotic cell death. The study demonstrated that apoptotic cell death of H22 - IL-2 cells was induced by the IL-2 gene modification. It may be one of mechanisms of decreased tumorigenicity of IL - 2 gene modified tumor cells.
