Cloning of cDNAs Encoding Extra-cellular Domain of Human IL-6, IL-10, IL-13 and SCF
- VernacularTitle:RT-PCR克隆人IL-6、IL-10、IL-13和SCF等四种细胞因子膜外区cDNA
- Author:
Zhongbin LIU
;
Zhigang TIAN
;
Rui SUN
- Publication Type:Journal Article
- Keywords:
cytokine;
cDNA cloning;
RT-PCR
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Human IL-6,IL-10,IL-13 and SCF cDNAs encoding extra - cellular domain were isolated by reverse transcription polymerase reaction (RT-PCR). These cDNA fragments were inserted into expression vector PBV220 which contains PRP_(L), promoters and SD sequence. Then the E. coli DH5? were tronsformed with the recombinant plasmids. After screening, the clones carrying those recombinant plasmids were identified. SDS-PAEG and biological assay indicated that human IL-6, IL-10 and IL-13 were expressed in E.coli. The expression level of rhIL-6 was as high as 30% of the total bacterial protein.
