DRB PCR-Single-Strand Coaformation Polymorphism Analysis of HLA-DR
- VernacularTitle:HLA-DRB PCR-单链构象多态性分析
- Author:
Jioncai LAN
- Publication Type:Journal Article
- Keywords:
HLA-DR;
PCR (Polymerase Chain Reaction) SSCP (Single-Strand Conformation Polymorphism)
- From:
Chinese Journal of Blood Transfusion
1988;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
DRB alleles of 10 different HLA-DR individuals tested by serology typing were studied with polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis, single-strand DNA of different sequence exhibited specific band patterns and mobilities by this technique. Our results suggested that PCR-SSCP is expected to be a useful tool for donor-recipient DRB allele HLA-matching in organ transplantation, as well as identification of criminal in forensic medicine.
