Studies on the Mechanisms of the Apoptotic Cell Death of Target Cells Mediated by Human Monocytes
- VernacularTitle:凝集素美洲商陆(PWM)依赖的人单核细胞介导的肿瘤细胞凋亡
- Author:
Haidong DONG
;
Zhenchao QIAN
- Publication Type:Journal Article
- Keywords:
pokeweed mitogen;
monocyte;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The mechanisms of cytotoxicity mediated by pokeweed-mitogen (PWM)-activated human peripheral blood monocytes was investigated in this study. By DNA electrophoresis and propidium iodide ( Pl) -DNA staining flow cytometry, the study demonstrated that apoptotic cell death of target U937 cells and Raji cells was induced in lectin-depen-dent monocyte-mediated cytotoxicity ( LDMC) . The LDMC-mediated DNA fragmentation in U937 cells could be completly inhibited by anti-TNF-?monoclonal antibodies McAbs, instead of the addition of monosaccharide (N-acetyl glucosamine, Glc NAC) . In contrast, Glc NAC inhibited the DNA fragmentation in Raji cells induced by PWM-monocytes while the anti-TNF-a McAbs had no effect. By flow cytometry, we also demonstrated that PWM could bind with tumor cells as well as with monocytes, at the stimulation of the production of nitric oxide ( NO-) from monocytes. This NO-production was enhanced in the presence of target cells, but the enhacement was abolished by the treatment of GlcNAc.The presence of Lectin-like receptors on the surface of monocytes and tumor cells may bring the effector target cells together, thus facilitating the induction of apoptosis in target cells by triggering the production of cytolytic factors and the modification of target cell surface antigens.
