Application of MTT Colorimetric Assay in Research of Cytokine Activity and Cytotoxicity of Killer Cells
- VernacularTitle:MTT法在检测细胞因子与细胞毒效应中的应用
- Author:
Zhigang TIAN
;
Jianhua ZHANG
- Publication Type:Journal Article
- Keywords:
MTT colorimetric assay;
cellular cytotoxicity;
cytokines;
lymphocyte proliferation;
tumor cell chemosensitivity
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
We used MTT assay to test the cellular cytotoxicity ( NK, LAK, CTL, Macrophage), cytokine activities ( 1L-1, 1L-2, 1L-6, TNF), proliferation of lymphocytes and chemosensitivity of tumor cells, and compared it with radioactive isotope assay. The results showed that the MTT assay may be used to test the cellular cytotoxicity, cytokine ac-tivity, proliferation of lymphocytes and chemosensitivity of tumor cells. We think it is a simple, rapid, economic and safety method.
