Study of Quality of Human Immunoglobulin Separated by the Rivanol-Cold Ethanol Combination Method(R-E)
- VernacularTitle:利凡诺—低温乙醇结合法分离的人血免疫球蛋白质量研究
- Author:
Lihua JIAO
- Publication Type:Journal Article
- Keywords:
Rivanol-cold ethanol combination method;
Immunoglobulin;
Quality
- From:
Chinese Journal of Blood Transfusion
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
A comparative observation of the quality of human immunoglobulin separated by the Rivanol-coldethanol combination method(R-E),the cold ethanol method(E)and the Rivanol-Ammoniumsulphate method(R-As)was performed.The experimental data and control data are as follows:Purityof immunoglobulin:96.67?0.93%(R-E,n=10);89.20?5.45%(E,n=8)and 96.22?0.67%(R-As,n=6).Proportion of IgG,IgA and IgM:89.26?0. 81%,6.29?0.38% and 1.16?0.28%(R-E,n=10);88.59?4. 87%,0.34?0.21% and 0.15%?0.09%(E,n=8)and 88.90?1.46%,7.08?1.28% and 0.24?0.29%(R-As,n=6)respectively.Proportion of IgG monomers and dimers,polymers,and fragments:95.96?1.38%,3.04?0.73% and 1.02?1.04%(R—E,n=7)and 98.43?0.57%,0.96?0.25% and 0.62?0.33%(E,n=3)respectively.All the preparations separated bythe three methods have been heated for 4 hours at 57℃,with their appearance being unchanged.
