Development of Monoclonal Antibody to Human H Blood Group Substance
- VernacularTitle:抗人H血型物质单克隆抗体的研制
- Author:
Lei YU
- Publication Type:Journal Article
- Keywords:
H blood group substance;
Monoclonal antibody;
Blood typing
- From:
Chinese Journal of Blood Transfusion
1988;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
A hybridoma cell strain 6H_3(a kind of IgG_1)which could secrete the monoclonal antibody to human H blood group substance was yielded through PEG—fusion of the SP2/0 cells and BALB/C mouse spleen cells immunized by H blood group substance. The agglutination titers of cell culture supernatant and the ascites fluid were 512 and 16384,respectively. Based on hemagglutination specificity test,absorption and eluate test,and hemagglutination inhibition assay,it was showed to be specific only for the human H blood group substance. The ability of hybridoma cells to secrete the monoclonal antibody wasn't be changed when it has been stored in the liquid nitrogen for a year. The agglutination activity of monoclonal antibody has remained stable when it has been kept at 56℃ for 30 min,and repeatedly subjected to freezing and thawing tests at —20℃ and 37℃,respectively. It can be expected to replace the traditional anti-H reagents and to be used for clinical blood typing.
