ANALYSIS OF PROTEINS AND ANTIGENS IN NINE STRAINS OF TOXOPLASMA GONDII BY MEANS OF SDS-PAGE AND IMMUNOBLOTTING
- VernacularTitle:应用聚丙烯酰胺凝胶电泳及免疫印迹技术分析弓形虫蛋白质与抗原的研究
- Author:
Changbin JIANG
;
Huizhen YANG
;
Heping YANG
;
Yang YANG
;
Keji XU
- Publication Type:Journal Article
- Keywords:
SDS-PAGE, immunoblotting, Toxoplasma strains
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The proteins and antigens of nine strains of Toxoplasma gondii were analyzed by SDS-PAGE and immunoblotting. These strains including RH strain, Zs-2 strain, CN strain and SHI, SH2, SH3, SH4, SH5, SH8 strains, were obtained from other laboratories or isolated from deformity fetus in our laboratory. SDS-PAGE analysis revealed that these strains were very close in the major bands of proteins and differences were observed only in part. There were common components in antigens of all strains using IgG antibody prepared from high-titre rabbit antisera raised against RH strain of Toxoplasma.
