Immunoaffinity Chromatography Method for Purification of Human Protein C
- VernacularTitle:免疫亲和层析法纯化蛋白C
- Author:
Hailing WANG
- Publication Type:Journal Article
- Keywords:
Protein C;
Monoclonal Anibody;
Immunoaffinity chromatography;
Calcium Ion
- From:
Chinese Journal of Blood Transfusion
1988;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
A special monoclonal antibody(McAb)to protein C(PC)was prepared,itrevealed the different conformation in the absence and presence of Ca~(++).This McAbwas coupled to Sepharose-4B and the gel was loaded into the column for thepurification of PC.The immobilized antibody was capable of binding PC fromthe plasma in the absence of Ca~(++).This immunopurification resulted in a 12 000-fold after one step proceduce.The specific activity of the PC product ranged be-tween 180-230 U/mg and behaved as two bands(?-PC and?-PC)on nonreducedSDS-PAGE and five bands(?-S-PC,?-S-PC,?-heavy chain,?-heavy chainand light chain)on reduced SDS-PAGE.
