PRELIMINARY STUDY ON THE ROLE OF MACROPHAGE IN PROCESSING AND PRESENTING ANTIGENS OF SCHISTOSOMA JAPONICUM
- VernacularTitle:巨噬细胞加工与呈递日本血吸虫抗原的作用
- Author:
Xihua FANG
;
Guan RONG
- Publication Type:Journal Article
- Keywords:
Macrophage,antigen processing,Schistosoma japonicum,Western blot.
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The horseradish peroxidase labeled affinity purified mice anti-UEA (Urea soluable egg antigen of Schistosoma japonicum) antibody was used in enzyme-linked immunoe ectrotransfer blot (EITB) te monitor the changes after UEA being processed by M?.The immune respon-sive peptides were delected in the culture supernatant and homogenate of M? pulsed with UEA in vitro (M?+).After processing by M? the high molecular weight UEA was cleaved into low molecular weight peptides,as shown,by the reactive bands.They markedly differed from that native UEA or trypsin-digested UEA; The bands of M? supernatant and homogenate showed similarity with certain quantitative differences.According to the result described above,we considered: 1.UEA could be processed into smaller pieces by M?,the style of processing is cleavage.2.The processed peptides might be released to extracellular environment.
