OBSERVATION OF ULTRASTRUCTURE AND DRUG SENSITIVITY OF IN VITRO CULTURED EXOERYTHROCYTIC FORM OF PLASMODIUM BERGHEI
- VernacularTitle:体外培养的伯氏疟原虫红外期超微结构及其药物敏感性观察
- Author:
Bin YANG
;
Hanying YAN
;
Weina PAN
- Publication Type:Journal Article
- Keywords:
Plasmodium berghei,exoerythrocytic form,in vitro cultivation,ultrastructure,drug sensitivity.
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The infrastructure of in vitro cultured exoerythrocytic stage (EE) of Plasmodium ber-ghei (P.b.)was observed under transmission electron microscope (TEM).The drug sensitivity of EE was also measured in vitro.The EE was cultured in monolayer host cell,fixed and embeded in situ.The ultrathin sections were prepared and examined by routine methods.The TEM pictures showed that the fine structure of in vitro cultured EE was similar with that of EE grown in rat hepatocytes in vivo,as described by Meis et al.The parasite was found within a parasitophorous vacuole,with nucleus,mitochondria,endoplasmic reticuia and Golgi apparatus were observed inside the parasite.After 24h cultivation,the EE was incubated for 48h in medium containing a serial concentrations of primaquine or chlo-roquine,then examined under light microscope.The percentage of abnormal parasites was calculated.The preliminary results showed that the sensitivity of cultured P.b.EE to primaquine and chloroquine was significantly different.At the same concentration of 1?10-5 mol/L,the percentage of abnormal parasites was 38.5?3.9% and 5.7?1.9% ,respectively These results demonstrated that the in vitro cultivation system of P.b.EE would have potential utility in antimalarial drug research.(Figs.1-6)
